Author + information
- Zubair Shah,
- Tanner Buehler,
- Lei Chen,
- Robert Vincent,
- Guangmimng Cheng,
- Bhaskar Das and
- Buddhadeb Dawn
Background: Synthetic boron based hepatocyte growth factor memetic (HGF-m) may have a ischemic preconditioning effect on cardiomyocytes.
Methods: Freshly isolated adult cardiomyocytes (ACM) were incubated overnight with/or without different concentrations of HGF-m. The cells were then subjected to hypoxia and cultured in air to simulated reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay and Western blotting (WB) analysis was also performed. H9c2 cells were pretreated, followed by hypoxia/reperfusion (H/R). The apoptosis rate was than detected by flow cytometry.
Results: H9c2 cells pretreated with HGF-m (H-2=0.01 μmol, H-3=0.05 μmol, H-4=0.1 μmol and H-5=0.5 μmol) and subjected to H/R, demonstrated a reduction in the apoptosis rate when compared to untreated (H-1) group (8.3%±2.2% in H-2, 11.5%±3% in H-3, 5.5%±1.3% in H-4 and 4.7%±0.8% in H-5; p<0.5). TUNEL assay revealed that ACM pretreated with HGF-m 0.05 μmol, and 0.1 μmol and subjected to H/R revealed significantly lower number of apoptotic cells (17%±3% and 14%±2% receptively when compared to untreated group 26%±5%; p<0.5). WB analysis revealed that ACM pretreatment results in lower expression of cleaved caspase-3 and higher levels of p-Met, Bcl-2, P-AKT and P-STAT3 expression.
Conclusions: HGF-m molecule has ischemic preconditioning/anti-apoptotic effect on ACM via pathways involving p-AKT and p-STAT3
Poster Hall, Hall C
Friday, March 17, 2017, 10:00 a.m.-10:45 a.m.
Session Title: Interventional Cardiology: Translation and Pre-Clinical Research
Abstract Category: 25. Interventional Cardiology: Translation and Pre-clinical Research
Presentation Number: 1116-194
- 2017 American College of Cardiology Foundation