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Background: It is known that changes of intestinal microbiota affect cardiovascular function in the context of metabolic syndrome, while the underlying mechanisms are still not clear. In this study we explored the possible link between host intestinal function, microbiota and cardiovascular function.
Methods: Mice were ovariectomized (OVX) to induce estrogen related metabolic syndrome and cardiovascular defect. Microbiota was analyzed by 16SRNA sequencing. Intestinal and fecal miRNA155 or let-7g was analyzed by qPCR. Cardiovascular function was analyzed by echocardiography. Colon specific delivery of miRNA candidates were achieved by oral gavage of Eudragit S functionalized microbubbles. Differences and correlations were analyzed by SPSS.
Results: In comparison with the sham-operated group, OVX mice showed significant lower percentage changes of lumen diameters in common carotid artery(CCA), left ventricle eject fraction and fractional shortening, but higher CCA thickness. Meanwhile, miR155 and let-7g were significantly upregulated in the intestinal epithelium and thus the feces. In vitro study with Cy3 labeled miRNA mimics revealed that these miRNAs could be possibly endocytosed by bacteria in the intestine. Delivery of miR155/let7g by Eudragit S functionalized microbubbles shaped the microbiota in a similar pattern as the OVX induced changes, together with altered cardiovascular function.
Conclusions: This study has established a possible regulatory axis of intestinal miRNAs-microbiota-cardiovascular function in the OVX model. Colon specific delivery of therapeutic miRNAs would possibly shape the microbiota towards better cardiovascular function in the context of metabolic syndrome.
Poster Hall, Hall C
Friday, March 17, 2017, 10:00 a.m.-10:45 a.m.
Session Title: Interventional Cardiology: Translation and Pre-Clinical Research
Abstract Category: 25. Interventional Cardiology: Translation and Pre-clinical Research
Presentation Number: 1116-196
- 2017 American College of Cardiology Foundation