Author + information
- Adnan Sultan,
- Yuting Zheng,
- Patrick J. Trainor,
- Siow Yong,
- Alok R. Amraotkar,
- Bradford G. Hill and
- Andrew P. DeFilippis
Background: Collagen is a major determinant of atherosclerotic plaque stability and prolidase is a rate-limiting enzyme in the breakdown and formation of collagen. We sought to determine prolidase activity at the time of acute myocardial infarction (MI) as compared to quiescent state and to patients with stable coronary artery disease (CAD).
Methods: Serum prolidase activity was measured in 49 patients with acute MI, of which a subset had clearly defined thrombotic MI (n=22) or non-thrombotic MI (n=12), and 15 patients with stable CAD. Serum prolidase activity was compared across study time-points [acute (T0 and T6h) and quiescent (Tf/u, >3mo) post MI or cardiac catheterization] and between acute MI subjects and stable CAD subjects. To identify covariates that were associated with prolidase activity (potential confounding factors), the signed Pearson correlation coefficient was computed for relevant covariates at the quiescent phase.
Results: Mean prolidase activity was lower at acute phase time points (T0, T6) versus quiescent phase (Tf/u) (RM-ANOVA time-point p<0.0001) in acute MI (both thrombotic and non-thrombotic) and stable CAD subjects. This pattern of change in prolidase activity was not different between acute MI and stable CAD subjects (p=0.35) or acute thrombotic and non-thrombotic MI subjects (p=0.28). Prolidase activity was significantly correlated with diabetes (R2=0.1, p=0.03). Mean prolidase activity was lower among diabetic acute MI subjects compared with acute MI subjects without diabetes and stable CAD subjects with and without diabetes at all time points. Prolidase activity was lower at the acute time points (T0, T6) compared with the quiescent state (Tf/u) (RM-ANOVA time-point p<0.0001) regardless of diabetes status.
Conclusions: Prolidase activity is not significantly different in patients with acute MI or stable CAD; however, prolidase activity is lower at the time of cardiac catheterization as compared to quiescent state follow-up. Prolidase activity is also lower in diabetic patients with acute MI, a finding which warrants further studies to determine if this is related to the greater risk of initial and recurrent MI in patients with diabetes.
Poster Hall, Hall C
Saturday, March 18, 2017, 3:45 p.m.-4:30 p.m.
Session Title: Emerging Basic Science in Ischemic Heart Disease
Abstract Category: 1. Acute and Stable Ischemic Heart Disease: Basic
Presentation Number: 1251-298
- 2017 American College of Cardiology Foundation