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Background: Myocardial injury caused by ischemia followed by reperfusion mediates a complex series of inflammatory response. The objective of this study was to investigate whether vitamin D has some protective effect on heart after myocardial I/R, and the mechanistic pathway of this effect.
Methods: A thirty two adult (4 – 6 month) male Albino-Webster mice were randomly divided into 4 groups: (1) sham group, (2) ischemia and reperfusion (IR) controlled operated group, (3) vehicle-treated group, and (4) vitamin D-treated group receiving vitamin D 1 mcg/kg once daily shortly before IR, hemodynamics and Evan blue staining were applied to evaluate cardiac function and area at risk (AAR). ELISA applied to investigate myocardial and plasma expression of cytokines (IL-1β, IL-6, and TNF-α),chemokine (MCP-1), and cTn-I and pERK1-2 was analyzed by Western blot. Further, the ischemia changes and myocytes injured were examined by Hematoxylin and Eosin (HE) stain.
Results: The results demonstrated that treatment of vitamin D markedly improved left ventricular function (LVF) in mice, and reduced plasma level of cTn-I as marker of cardiac injury. Moreover, the effects of vitamin D was associated with attenuations in both chemokine and cytokines expression following I/R, that accompanied by down-regulation of activation of ERK1-2 pathway.
Conclusions: The treatment with vitamin D was able to improve LV function after I/R, which is associated with reductions the activity of pERK1-2 as mechanistic of its action.
Poster Hall, Hall C
Saturday, March 18, 2017, 9:45 a.m.-10:30 a.m.
Session Title: Cutting Edge Vascular Medicine
Abstract Category: 38. Vascular Medicine: Basic
Presentation Number: 1205-358
- 2017 American College of Cardiology Foundation