Author + information
- Lilei Yu,
- Guannan Meng,
- Bing Huang,
- Zhuo Wang,
- Songyun Wang,
- Menglong Wang,
- Xuefei Li,
- Liping Zhou,
- Shenxu Yuan,
- Yuhong Wang,
- Xiaoya Zhou and
- Hong Jiang
Background: Evidences show that gut microbes and their products could activate the autonomic nervous system, which plays an important role in the modulation of atrial electrophysiology and arrhythmogenesis. Trimethylamine N-oxide (TMAO), a gut microbes-derived metabolite, has been proved to be associated with cardiovascular diseases. The present study aimed to investigate the role of TMAO, a gut microbes-dependent metabolite, in the progression of early stage of atrial fibrillation (AF) induced by rapid atrial pacing (RAP).
Methods: Twenty one anesthetized open-chest dogs were assigned to three groups: TMAO group (n=9, TMAO injection into 4 major ganglionated plexis (GPs) plus 6 hours of RAP), saline group (n=6, saline injection into 4 GPs with 6-hour RAP), and control group (n=6, saline injection into 4 GPs without RAP). Effective refractory period (ERP), window of vulnerability (WOV), anterior right ganglionated plexi (ARGP) function and neural activity were recorded. At the end of the experiment, expression of inflammatory factors, c-fos, NGF, and NF-kB in GPs were determined.
Results: Compared with the control group, 6 hours of RAP significantly decreased ERP, increased ERP dispersion, widened WOV, enhanced ARGP function and neural activity, increased NGF and c-fos expression, up-regulated inflammatory cytokines. All these alterations were further aggravated by TMAO injection. In addition, TMAO activated the NF-kB signaling in GPs when compared to control group and saline +RAP group.
Conclusions: TMAO could aggravate acute electrical remodeling induced by short-term RAP through exacerbating autonomic remodeling. Increased inflammatory cytokines in GP due to the activation of NF-κB signaling may contribute to these effects of TMAO.
Poster Hall, Hall C
Saturday, March 18, 2017, 3:45 p.m.-4:30 p.m.
Session Title: Arrhythmias and Clinical EP: Basic 4
Abstract Category: 4. Arrhythmias and Clinical EP: Basic
Presentation Number: 1238-112
- 2017 American College of Cardiology Foundation