Author + information
- Elena Gonzaleza,b,
- Judith Bernal-Ramireza,b,
- Andrea Cordero-Reyesa,b,
- Jerry D. Estepa,b,
- Keith Youkera,b,
- García-Rivas Gerardoa,b,
- Guillermo Torre-Amionea,b and
- Catedra de Cardiologiaa,b
Background: Pre-clinical studies have demonstrated that elevated cytokines are associated with worsening of the heart failure (HF) state. The association of cytokines such as IL-18 with markers of cardiac injury is not well established. Therefore we aim to determine if IL-18 is present during an acute decompensated HF (ADHF) episode and correlates with mortality and cardiomyocytes dysfunction.
Methods: To determine the effect of IL-18 on cardiomyocytes function, rat cardiomyocytes were isolated and cultured in presence or absence of 10ng/ml of IL-18. Calcium handling and cell shortening was analyzed by confocal microscopy. Serum samples from patients (105) with ADHF were obtained within 24 hours of admission and cytokine pro-inflammatory profile (INF- γ, IL-12, IL-1β, IL-2, TNF-α, IL-4, IL-5, IL-6 and IL-18) was analyzed using a Procarta multiplex cytokine panel assay. Data was retrieved from patient charts and the primary endpoint was mortality within 1 year.
Results: At cellular level, despite cardiomyocytes function remains unaltered in presence of IL-18 it potentiates the impairment observed by TNF- α. IL-18 decreased the calcium transient amplitude in 41.5% and increased the time to 50% decay in 73.7%, as consequence the cell shortening decrease by a 62.9%. In addition, measurable levels for the following cytokines IL-18, INF-γ, IL-6, IL-1 β were obtained, with an increased levels of IL-18 in patients who died (p-value <0.0001). There was a significant correlation between IL-18 and troponin I levels (r=0.8, p-value= 0.0001).
Conclusions: IL-18 is a cytokine related to increased inflammation with a pro fibrotic and hypertrophic effect in the heart. We observed that IL-18 was also associated with increased mortality in patients with ADHF probably by the negatively modulation on excitation contraction coupling. In addition, IL-18 positively correlates with Troponin I reinforcing the idea that IL-18 might add prognostic value in HF patients especially in risk of death.
Poster Hall, Hall C
Friday, March 17, 2017, 3:45 p.m.-4:30 p.m.
Session Title: Diabetes and Endothelial Dysfucntion in Heart Failure
Abstract Category: 12. Heart Failure and Cardiomyopathies: Basic
Presentation Number: 1162-249
- 2017 American College of Cardiology Foundation