Author + information
- Received April 23, 1985
- Revision received September 30, 1985
- Accepted October 7, 1985
- Published online February 1, 1986.
- Pramod K. Mohanty, MD, FACCa,
- James R. Sowers, MD,
- Marc D. Thames, MD, FACC,
- Fran W.J. Beck, MS,
- Akira Kawaguchi, MD and
- Richard R. Lower, MD
- ↵aAddress for reprints: Pramod K. Mohanty, MD, Cardiology Division, Veterans Administration Medical Center, Richmond, Virginia 23249.
Myocardial norepinephrine is markedly reduced after cardiac transplantation because of interruption of postganglionic cardiac sympathetic nerves. There are also substantial stores of dopamine in the myocardium, but the influence of cardiac denervation on dopamine remains unknown. The effect of cardiac transplantation was determined and, thus, the effect of denervation on myocardial norepinephrine, dopamine and epinephrine. Myocardial catecholamines were measured with highperformance liquid chromatography with electrochemical detection in five dogs 6 to 8 weeks and in four dogs 8 to 12 years after cardiac autotransplantation and in six sham-operated dogs with intact cardiac innervation.
Norepinephrine, dopamine and epinephrine levels were determined from samples obtained from the right and left atria and ventricles. Samples from the left ventricular apex and base were analyzed separately. There was a striking depletion of norepinephrine in all cardiac chambers after short-term autotransplantation. The norepinephrine content of the left atrium in sham-operated dogs (1,659 t 219 ng/g) was significantly higher than that of dogs with long-term autotransplanted hearts (754 ± 372 ng/g). Sham-operated dogs and dogs with long-term autotransplanted hearts had statistically significant (p < 0.05) differences in norepinephrine content in the left ventricular apex (480 ± 197 versus 294 ± 198 ng/g), left ventricular base (876 ± 2204 versus 654 ± 156 ng/g) and right ventricle (766 ± 133 versus 247 ± 29 ng/g).
In contrast to norepinephrine, dopamine concentrations were relatively preserved in the short-term group despite the virtual depletion of myocardial norepinephrine. The data confirm that cardiac denervation depletes myocardial norepinephrine in all portions of the transplanted heart and that norepinephrine levels remain depressed up to 12 years after transplantation. The data also demonstrate that dopamine levels are only modestly decreased compared with norepinephrine levels (p = NS).
This study was supported by Grant HL30506 from the National Heart, Lung, and Blood Institute, Bethesda, Maryland and by funds from the Veterans Administration, Washington, DC. This report was presented in part at the American Federation of Clinical Research National Meeting, Washington, D.C., May 1984 and the 34th Annual Scientific Sessions of the American College of Cardiology, Anaheim, California, March 1985.
- Received April 23, 1985.
- Revision received September 30, 1985.
- Accepted October 7, 1985.
- American College of Cardiology Foundation