Author + information
To search the distinctive miRNA profiles in plasma of the elderly patients with unstable angina (UA) and stable angina (SA), and to find more effective markers of UA in elderly people.
We compared the miRNA expression levels in plasma samples from 10 elderly patients with UA and 10 elderly patients with SA by using microarray-based miRNA chip, then validated by Real-time PCR in 30 UA patients and 30 SA patients. Using Mirbase, Miranda, and Targetscan to predict the selected miRNA target genes.
Six miRNAs (i.e.,mir-21-5p, mir-1202, mir-1207-5p, mir-1225-5p, mir-3162-3p, let-7f-1-3p) were selected in screening step. Mir-1202, mir-1207-5p and mir-1225-5p showed a statistically significant down-regulation (p<0.05), while mir-3162-3p showed up-regulation (p<0.05) in confirmation step. Conversely, mir-21-5p and let-7f-1-3p were not confirmed as significant at this step. Of all single miRNAs, miR-3162-3p showed the highest discriminatory power in the diagnosis of elderly patients with UA (AUC: 0.79, 95%CI: 0.675-0.905). The discriminatory power of a panel of three miRNAs (mir-3162-3p/ mir-1225-5p/ mir-1207-5p) was highest, with an AUC of 0.91 (95% CI: 0.84–0.98), followed by mir-3162-3p/mir-1225-5p (AUC: 0.833, 95% CI: 0.732-0.934) and mir-3162-3p /mir-1207-5p (AUC: 0.817, 95% CI: 0.712-0.922).
Multi-miRNA panel could provide higher diagnostic value for the diagnosis of elderly patients with UA. MicroRNAs, such as mir-3162-3p/mir-1225-5p/mir-1207-5p, mir-3162-3p/mir-1225-5p may become targets for diagnosis of elderly patients with UA.