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Corydalis hendersonii Hemsl. (CH) has been used for centuries in China largely for the treatment of high altitude polycythemia, hypertension, hepatitis, edema, gastritis, and other infectious diseases, but never employed for coronary heart diseases. The current study is to investigate the cardioprotective effects of CH extract in an ICR mouse model of myocardial ischemic injury.
CH ethanol extract was orally administrated to AMI mice that was established by ligation of the left ventricular dysfunction (LAD) coronary artery. Mice were randomly divided into six groups (n = 12 per group): sham group, model group, CH groups treated with three doses of CH (100‒400 mg/kg, intragastrically), and captopril group (positive control, 16.67 mg/kg, intragastrically). Measurement by echocardiography of ejection fraction (EF) and fractional shortening (FS) to evaluate the heart function. Inflammatory cell infiltration in collagen deposition in the myocardial ischemic heart tissues were observed by histopathological examination. Creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) levels in serum, AngII, TNF-α, and IL-6 in plasma, and expressions of proteins MMP-2 and MMP-9, expressions of signal-transduction proteins, p65, IκBα, JAK2, and STAT3 were measured by ELISA and Western blot analyses.
A dose-dependent cardioprotective effect was observed by CH treatment in AMI mice. As compared to those in the model group, LVEDs and LVEDd were significantly reduced, and EF and FS were improved in CH groups; inflammatory cell infiltration in the myocardial infarct was attenuated in all CH treated groups. Serum levels of CK-MB and LDH were decreased; expressions of AngII, TNF-α, and IL-6 in plasma were reduced, and MMP-2 and MMP-9 expressions, expressions of p-p65, p-IκBα, p-JAK2, p-STAT3, MMP-2, and MMP-9 in AMI mice were decreased.
CH extract showed a cardio protective effect against myocardial ischemic injury in an anti-inflammation and anti-myocardial fibrosis way. The present finding is the first discovery of cardio protection of CH against AMI injury for the first time.