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To assess the changes in the content of proinflammatory markers and the functional state of mitochondria in patients with chronic heart failure (CHF) under the influence of therapy with coenzyme Q10.
104 patients with chronic heart failure II-III functional classes were included into the study. Patients of group 1 (51 patients) received standard therapy of CHF, 2 groups received additionally coenzyme Q10 120 mg per day for 3 months. Initially, after 3 months of therapy in the blood serum: the content of interleukins Il-6 and Il-10 (by the ELISA method), the highly sensitive C-reactive protein (hs-CRP) (immunoturbidimetric method), the concentration of the N-terminal pro-brain natriuretic peptide -proBNP) (electrochemiluminescent method). A pilot study of the functional state of mitochondria was carried out by determining the membrane potential of mitochondria and the permeability of the cell membrane of peripheral blood mononuclear leukocytes by flow cytometry (10 patients in 2 groups). The analysis of the results was carried out using Kaluza ™ software (Beckman FC-500, USA). Statistical analyzes were performed with Statistica 10.0.
The content of NT-proBNP after 3 months of treatment significantly decreased in groups 1 and 2: in group 1 from 490.7 (250.5; 752.9) pg / ml to 134.6 (80.8; 202.1) pg / ml (p <0.05) and in the 2nd group with 701.3 (271.4; 1385.5) pg / ml to 230.8 (178.9; 443.4) pg / ml (p <0.05). Similarly, at 3 months of therapy, both Il-6 and hs-CRP (p <0.05) were observed in both groups. Initially, in patients with CHF, the mononuclear leukocyte population contained only 65.4% of DiOC-positive cells and 33.5% of DiOC-negative cells (early stage of apoptosis). On the background of treatment in patients of the 2 groups of DiOC-positive cells, 72.8% were determined, and the content of DiOC-negative cells decreased to 26.7%. A study of spontaneous apoptosis after a 12-hour incubation of a leukocyte suspension at a temperature of + 37 ° C showed that the mononuclear leukocyte population obtained from the peripheral blood of patients before the treatment contained 34.7% DiOC-positive cells, DiOC-negative cells - 59%. After the course of therapy, the percentage of DiOC-positive cells was 52.9%, and DiOC-negative cells - 43%.
The effect of therapy in patients with CHF showed a decrease in the content of proinflammatory laboratory markers. The increase in DiOC-positive cells, including after 12-hour incubation, is probably due to the persistence of a high proton concentration in the mitochondria, an increase in their functional activity and the stability of the mitochondrial membrane against a background of therapy involving coenzyme Q10.