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To investigate the expression and the effect of connective tissue growth factor (CTGF) on rheumatic myocardial fibrosis of rheumatic heart disease (RHD).
The papillary muscles samples were obtained from patients with RHD during mitral valve replacement. The expression of CTGF mRNA and CTGF protein were detected with semiquantitative RT-PCR technique and immunohistochemistry technology in the papillary muscles cell from 38 RHD patients and 20 normal papillary muscles samples. The area of myocardial fibrosis was measured by imaging analysis system. Analysis the relationship between the expression of CTGF and the area of myocardial fibrosis.
Compared with normal controls, the mean levels CTGF mRNA expression in the papillary muscles samples of the patients were significantly increased, RT-PCR amplication production Reinhoit Zahl (RZ) (0.8229±0.2697), normal controls (0.0813±0.0363), (P<0.01). In immunohistochemistry study CTGF protein was strongly expressed in the papillary muscles samples were obtained from patients, whereas it was low expressed in normal controls(43.107±5.207, 2.661±0.946, P<0.01). The expression of CTGFmRNA and CTGF protein in papillary muscles of RHD were all correlated positively with the area of myocardial fibrosis (r =0.889, 0.865, P<0.01). The expression of CTGFmRNA in papillary muscles of RHD was correlated positively with the expression of CTGF protein (r =0.823, P<0.01). The expression of CTGFmRNA, CTGF protein in papillary muscles of RHD didn’t show the significantly correlation with the case history, sex and age, but correlated with remarkably with cardiac functional gradings.
CTGF expression in the papillary muscles of the RHD patients is significantly increased, which suggests CTGF may play an important role in myocardial fibrosis of RHD.