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To evaluate the capacity of localized delivery of recombinant adenovirus carrying exogenous chemokine stromal cell-derived factor(SDF-1α) and differentiation factor bone morphogenetic protein(BMP2) to the acute myocardial infarction rat model by ultrasound-targeted microbubble destruction(UTMD).
100 healthy SD rats were randomly divided into 10 groups: sham operation group, myocardial infarction group, ultrasound+myocardial infarction group, microbubble+myocardial infarction group, ultrasound+microbubble+myocardial infarction group, ultrasound+microbubble+myocardial infarction+pAd-EGFP/SDF-1α group, ultrasound+microbubble+myocardial infarction+pAd-RFP/BMP2 group, ultrasound+microbubble+myocardial infarction+pAd-EGFP/SDF-1α+pAd-RFP/BMP2 group, then according to the different compatibility ratio of pAd-EGFP/SDF-1α and pAd-RFP/BMP 2, this group was divided into three subgroups: 1:1, 1:2 and 2:1. Ligation of the left anterior descending at the 6th hours after myocardial infarction, through UTMD, the exogenous SDF-1α, BMP2 and different combinations of SDF-1α and BMP2 were transfected into infarcted myocardium. On The 28th Day after treatment, the expression of green fluorescent protein(GFP) and red fluorescent protein(RFP) in the infarct, non-infarct and border regions were observed by confocal laser scanning microscope. Meanwhile, Western blot was used to measure the expression of EGFP/SDF-1α and RFP/BMP2 in the infarct, non-infarct and border regions.
There was significant difference in the expression of EGFP/SDF-1α and RFP/BMP2 among the non-gene carrying groups (P>0.05). According to the different kinds of gene-carrying, the expression of EGFP/SDF-1α and RFP/BMP2 were statistically significant among the gene carrying groups (P<0.05). Furthermore, the expression of EGFP/SDF-1α and RFP/BMP2 in the border regions was significantly different compared with the infarct and non-infarct regions (P<0.05).
This study showed that UTMD could promote the transfection of exogenous EGFP/SDF-1α and RFP/BMP2 into the hearts of AMI rats. Moreover, the optimal transfection in the border regions was better than that in the infarct and non-infarct regions.