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To investigate human venous blood in the preparation of mixed thrombus which platelets and fibrin for providing a stable and simple method for thrombolysis in vitro.
Venous blood was taken from healthy blood a donor that was per person 8-10ml, who had not been treated with anticoagulants or platelet function inhibitors within 2 weeks. The thrombus was prepared in vitro, then divided into four groups, venous blood (group A), venous blood+ADP+thrombin+CaCl2 (group B), venous blood+CaCl2 (group C) and intracoronary thrombus in acute myocardial infarction patients within 2 hours as control group (Group D). The platelet and cellulose in the thrombus were detected by HE staining, immunohistochemistry and scanning electron microscope. Extracorporeal circulation device was used to simulate 180mmHg to detect the stability of thrombosis. Recombinant tissue type plasminogen activator (rt-PA) was dissolved Thrombin vitro, and the lysis rates of thrombosis was calculated.
Compared with the control group, only the B group showed a large number of platelets and beam formation with HE staining, platelet aggregation distribution with immunohistochemical staining, a large number of platelets, fibrous reticular structures and few red blood cells were observed under scanning electron microscope. Under the extracorporeal circulation device of 180mmHg, the thrombus was not dissolved in 4 hours In the B group, but the A and C groups were almost completely dissolved within 1min under the pressure of 120mmHg, the lysis rate of the A and C groups were 35%±4% and 39%±6% compared with which were higher than B group 71%±5% in vitro (P<0.05).
The venous blood+ADP+thrombin+CaCl2 group is most likely to form mixed thrombus with platelets and fibrin, which has a better stability, higher cracking rate, thus, it can provide a stable and simple thrombus model for thrombolysis in vitro.