Author + information
- 1Department of Internal Medicine, Cardiology Section, College of Medicine, Aljouf University, Sakaka, Kingdom of SaudiArabia
- 2Department of Internal Medicine, Endocrine Section, College of Medicine, Aljouf University, Sakaka, Kingdom of SaudiArabia
- 3Department of Cardiology, Xiangya Hospital, Central South University, Changsha, Hunan, China
The purpose of this study was to explore the diagnostic value of cardiomyocyte-enriched circulating miR-208b, miR-499, miR-378 and miR-424 in acute coronary syndrome patient.
This study a single-center, prospective diagnostic study of acute coronary syndrome (ACS) patient. Fifty unstable angina patients, fifty NSTEMI patients, fifty STEMI patients and fifty healthy control subjects were included in our study (n=200). ACS patients were diagnosed according to ESC/ACCF/AHA/WHF guidelines and blood sample were collected within 12h of onset of chest pain. Real-time quantitative reverse-transcription polymerase chain reaction was used to determine the expression of miRNAs in plasma. To examine the potential diagnostic value of circulating miRNAs as a clinical biomarker for ACS patients, receiver operating characteristics curve (ROC) analysis was performed.
We analyzed the circulating expression levels of miR-208b, miR-499, miR-378 and miR-424 in ACS patients. We found that cardiac enriched plasma miR-208b and miR-499 levels were significantly increased in unstable angina (UA), NSTEMI and STEMI patients as compared with healthy subjects (P< 0.001). On the contrary, the expression levels of plasma miR-378 and miR-424 were markedly decreased in UA, NSTEMI and in STEMI patients than those with healthy subjects (P < 0.001). Receiver operating characteristic curve analysis revealed that the respective areas under the curve (AUC) for circulating miR-208b, miR-499, miR-378 and miR-424 were 0.979, 0.947 0.903 and 0.928 in UA patients, 0.981, 0.973 0.927 and 0.940 in NSTEMI patients, 0.984, 0.978 0.959 and 0.950 in STEMI patients, respectively, as compared with healthy subjects
Our results suggest that circulating miR-208b, miR-499, miR-378 and miR-424 may be used as a potential clinical biomarker for diagnosis of acute coronary syndrome patient.