Author + information
To create an experimental animal model of acute Stanford type A aortic dissection for researching its pathophysiologial changes and offer a proper animal model for clinical therapy.
Eighteen adult beagle dogs (weight, 8–12 kg) were used. The ascending aorta was exposed by a standard full-length sternotomy. The entry of the aortic dissection was created surgically just distal to the origin of the ascending aorta. Normal saline solution was injected into the aortic wall (ie, media) create the dissection, the remaining media and intima was incised transversely and the incision of the adventitia and part of media were then sutured and the blood bleeding from the false lumen at the entry point .
All of the 18 dogs, 3 died because of hemorrhagic shock .We created 15 models successfully, and all of the 15 surviving dogs had completely patent true and false lumina. The length of aortic dissection is 13.5～17.5cm (16.67±0.42cm). Microscopic examination showed that the dissection was created in the tunica media layer, making it identical to Stanford type A aortic dissection in humans.
In this animal model of Stanford type A aortic dissection, the false lumen has excellent long-term patency and the dissection plane is histologically similar to that in human. This model may contribute to the development of researching its pathophysiologial changes of Stanford type A aortic dissection and offer a proper animal model for clinical therapy.