Author + information
To study the mechanism and the relationship of the follistatin-like protein 1 and miRNA-200 in acute myocardial infarction (AMI).
80 AMI cases were enrolled as AMI group, and 80 non-AMI cases were recruited as control group. Plasma FSTL1 was detected by ELISA. FSTL1 related ncRNAs (miRNAs, lncRNAs, circRNAs) and their functions were predicted by bioinformatics, and hsa-miR-200b-3p was tested by qRT-PCR. Luciferase analysis wether miR-200b-3p regulated FSTL1. 20 SD rats were divided into AMI group and Sham group, AMI group rats were operated the left anterior descending coronary artery ligation, and Sham group were not ligated. Venous blood and heart tissue were collected postoperative 1d, plasma cTnI and FSTL1 expression were tested by ELISA, plasma and heart rno-miR-200b-3p were detected by qRT-PC, and FSTL1 protein levels were performed by Western blot.
There were no statistical differences in age, gender and history; total cholesterol and low density lipoprotein in AMI group were higher than the control group (P < 0.05); Plasma FSTL1 expression was significantly higher in AMI group (P>0.05), the ROC curve analysis found that the AUC was 0.98 (95% CI: 0.96-0.99, P < 0.05); bioinformatics found FSTL1 found mainly was related with miR-200 family (has-miR-200b-3p, has-miR-200c-3p and has-miR-429), its related lncRNAs were CTD-2630F21.1, CTB-92J24.2, RP11-473I1.10, C11orf95 and XIST, its related circRNAs were C2orf29_hsa_circ_000665, MYO9B_hsa_circ_001731 and SMAD2_hsa_circ_000030; GO, KEGG and PANTHER prediction found that MAPK, PI3K Akt, TGF beta, Oxidative stress response, Inflammation mediated by chemokine and cytokine and Apoptosis signaling pathway may relate to it; plasma has-miR-200b-3p expression was significantly lower than the control group (P < 0.05), and was negative correlation with FSTL1 (Pearson correlation coefficient is 0.66, P < 0.05). Luciferase analysis found that miR-200b-3p regulated FSTL1. AMI group rats plasma FSTL1 expression were increased significantly than the Sham group (P<0.05), the AUC is 1.000 (P<0.05); FSTL1 express both at the mRNA level and protein level were significantly lower in AMI rats (P<0.05) at postoperative 1d; plasma rno-miR-200b-3p expression were significantly decreased in AMI group (P<0.05), while heart tissue rno-miR-200b-3p expression were increased significantly in AMI rats (P<0.05).
FSTL1 may be associated with AMI, and can be used as a biomarker of myocardial injury, and may involve in inflammation, oxidative stress and cell apoptosis in AMI by hsa-miR-200b-3p mediated regulation.