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To investigate the role of Liver X α (LXRα) in homocysteine(Hcy) induced foam cells formation.
THP-1 monocytes were cultured and induced by ox-LDL to become to foam cell. Then cells were intervened by different concentrations of homocysteine(Hcy) and LXRα agonist T0901317 for 24 hours. Oil red O staining was performed for identification and qualitative analysis. The protein expression of LXRα and its target gene ABCG1 as well as ABCA1 was detected by cyto-immunofluorescence and Western blot.
Oil red staining showed that Hcy facilitate foam cell formation. The immunofluorescence and Western blot result suggest that the protein expression of LXRα, ABCG1 and ABCA1 were decreased after the intervention of Hcy and T0901317 could reverse this effect.
Our results suggesting that LXRα might be one of the key role of macrophage lipid metabolism interrupted by Hcy.