Author + information
- Linghui Lu1,
- Yong Wang2,
- Jing Wang3,4,
- Qiyan Wang2,
- Yi Zhang3,4,
- Qian Zhang2,
- Yan Wu5,
- Chun Li3 and
- Wei Wang1
- 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine
- 2School of Life Sciences, Beijing University of Chinese Medicine
- 3Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine
- 4School of Chinese Materia Medica, Beijing University of Chinese Medicine
- 5Center of Scientific Experiment, Beijing University of Chinese Medicine
To investigate the effect of QSG on inflammation-mediated myocardial apoptosis and to explore the underlying pharmacological mechanisms.
In vitro apoptotic model was induced by culturing H9C2 cardiomyocytes with supernatant of lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage. Anti-apoptosis effect of QSG was evaluated by cell viability assay, Hoechest33258 and Rhodamine123 staining. In vivo HF mice were induced by left anterior descending (LAD) coronary artery ligation after pre-administration of QSG or Celecoxib for 7 days. Heart functions were assessed by echocardiography and apoptotic cardiomyocytes were detected by TdT mediated dUTP Nick End Labeling (TUNEL) staining 24h after acute myocardial infarction (AMI). The critical inflammation mediators and proteins in apoptotic pathway were quantified by western-blot.
In vitro study showed that QSG increased cell viability, inhibited cell apoptosis, reduced the expressions of cyclooxygenase2 (COX2) and p-nuclear factor kappa B (p-NFκB), and down-regulated ratio of Bax/B-cell lymphoma-2 (Bcl-2). In vivo study showed that QSG improved heart function, ameliorated myocardial inflammation and inhibited apoptosis. Expression of COX2 was inhibited by QSG. Factors in apoptotic signaling pathways, including Bcl-2, Bax, P53, Fas ligand (FasL) and Caspases, were regulated by QSG.
QSG treatment attenuates inflammation-induced apoptosis in a multi-components and multi-targets mode. QSG might be an ideal alternative drug for inhibiting myocardial apoptosis in HF.