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Doxorubicin could damage macrophages, which might lead to the decrease of the phagocytic activity and influence the secretion of related cytokines. Some active components have been reported to inhibit the doxorubicin induced cell damage. In this study, we investigated the protective effects of ginsenoside Re on macrophages RAW 264.7 cells injury induced by doxorubicin.
RAW 264.7 cells were cultured at 37°C and 5% CO2 with DMEM culture medium which is containing inactivated bovine serum of 10%. Different concentrations of doxorubicin (0.01-100 μmol/L) were used as injured model on RAW 264.7. The optimal concentration of doxorubicin was screened and the damage model was established. Cells were co-treated with different concentrations of ginsenoside Re (0.01-100 μmol/L) for 24h with processing of the cell activity and anti-inflammatory effect detection.
The optimal concentration of RAW 264.7 induced by doxorubicin was 1 μmol/L with significantly cell viability decreasing. Compared with the normal group, there was no significant change in the activity of ginsenoside Re in different concentrations. Ginsenoside Re of different concentrations could prevent the injury induced by doxorubicin. Ginsenoside Re could increase the cell viability by MTT and LDH detection. The overexpression of TNF-α expression was also inhibited by ginsenoside Re. Ginsenoside Re concentration of 0.1 μmol/L showed the best protection effect.
Ginsenoside Re can protect the macrophages that were injured by doxorubicin. The better protective concentration of ginsenoside Re is 0.1 μmol/L.