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Danshen and Chuanxiong are the most frequently-used traditional herbal medicines to treat with cardiovascular diseases. Both of them have been reported to have anti-inflammatory effect. Although the combined use of Danshen and Chuanxiong are very common in clinic, the compatibility proportion of them has few reports. The purpose of this study is to explore the anti-inflammatory effect of different proportions of Tanshinone IIA and senkyunolide A, the main ingredients of Danshen and Chuanxiong in vitro.
RAW264.7 cells were cultured at 37°C and 5% CO2 with DMEM culture medium which is containing inactivated bovine serum of 10%. We use Chou-Talalay combination index equation to investigate the best ratio of Tanshinone IIA and senkyunolide A in anti-inflammation. Adriamycin (1 μmol/L) was use as model. Different concentrations of Tanshinone IIA (0.1-10 μmol/L) and senkyunolide A (0.1-10 μmol/L) were designed as a chessboard pattern. After administration of 24h, the cell activity and anti-inflammatory effect of each group was determined by MTT, LDH and TNF-α expression.
As drug concentration increased, the anti-inflammatory effect was increased in lower concentrations when two drugs used alone. The optimal concentration was about 1 μmol/L. In low concentration (0.1-1 μmol/L), Tanshinone IIA and senkyunolide A showed a synergistic trend in anti-inflammation which is better than high concentrations (1-10 μmol/L). When the two drugs were used together, the best ratio of Tanshinone IIA and senkyunolide A was 1:3-1:5.
Tanshinone IIA combined with senkyunolide A has synergistically anti-inflammatory effect in 0.1-1 μmol/L. The best ratio of the two medicines is 1:3-1:5 according to current research