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Obesity has become an epidemic disease in today’s world. In obese state, excessive fatty free acids (FFA) from hyperplastic adipose tissue were released to adipocytes, skeletal muscle and liver, which increases oxidative stress in adipocytes and results in insulin resistance. Ginsenoside Rb1 was showed anti-oxidant effect on H2O2-induced premature senescence in our previous studies. However, the effect of Rb1 on FFA-induced oxidative stress has rarely been demonstrated. This study is to testify whether Rb1 has a protective role on the oxidative stress after induction by FFA in 3T3-L1 adipocytes.
In this study, 3T3-L1 adipocytes were exposed to 0.5 mM FFAs with or without Rb1 at 10, 20, 40 μM. Endothelial nitric oxide synthase (eNOS) and superoxide dismutase 2 (SOD2) expression were detected by western blotting, intracellular reactive oxygen species (ROS) and nitric oxide (NO) production were measured as level of oxidative stress.
FFA decreased the expression of eNOS and SOD2 which was blocked by Rb1 in a dose-dependent manner. Rb1 also suppressed FFA-induced ROS production and increased NO contents, suggesting that the protective effect of Rb1 from FFA-induced oxidative injuries was at least partially achieved by up-regulation of antioxidant enzymes.
Ginsenoside Rb1 inhibits FFA-induced oxidative stress in 3T3-L1 adipocytes at least partially through up-regulation of eNOS and SOD2 expression.