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To study the preventive effect of Quercetin on silent mating type information regulation 2 homolog 1(SIRT1) and adenosine monophosphate-activated protein kinase (AMPK) in the rat with diabetics.
Male Wistar rats were divided into 4 group, normal control (n＝10),diabetic rats with high-fat diet (n＝10), diabetic rats with high-fat diet plus Quercetin (25 mg/kg·d, n＝10), diabetic rats with high-fat diet plus Quercetin (50 mg/kg·d, n＝10). the following indices of rats were measured respectively, Levels of blood creatine kinase (CK) and serum lactate dehydrogenase (LDH) as well as myocardial nonesterified fatty acids (NEFA) and collagen were determined using ultraviolet spectrophotometric, the concentration of myocardial fatty acid transport proteins (FATPs) and fatty acidβ-oxidase (FA-β-oxidase) were measured by ELISA method, the protein expression of NF-κB, silent mating type information regulation 2 homolog 1 (SIRT1) and adenosine mon ophosphate-activated protein kinase (AMPK) were detected by westernblot.
Levels of CK and LDH as well as NEFA were remarkably decreased after treatment of TSG. Quercetin caused a significant increase in concentration of myocardial FATPs and FA-β-oxidase in DM rat model. In diabetic group, Cardiac tissue SOD and CAT activities were significantly lower than control group (p＜0.05). Quercetin caused significant increase in the SOD and CAT activities of DM+ Quercetin groups cardiac tissue compared to DM group (p＜0.05). In diabetic group, Cardiac tissue NF-κB and MDA levels were increased compared to control group (p＜0.05), and groups of DM+ Quercetin had lower NF-κB and MDA levels than diabetic group (p＜0.05).Quercetin dramatically restored the decrease of SIRT1, AMPKαand pAMPKα protein expression in diabetic rats.
As a conclusion, based on the results we obtained from this study, we determined in diabetic rats with high-fat diet, increased glucose levels and cardiac damage markers decreased significantly, after administration of Quercetin, that oxidative stress and NF-κB levels increased while SIRT1 levels decreased in the diabetic group. These findings indicate that the protective mechanisms of Quercetin against diabetic rats are involved in the alleviation of Inflammatory mediator’s injury and energy metabolism.