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MiR-208b, a cardiac-specific miRNA, has been implicated in myocardial fibrosis by affecting the heterogeneity of myosin. This study was to evaluate the prognostic potential of miR-208b-3p in patients presenting with acute myocardial infarction (AMI).
A total of 140 AMI patients who underwent percutaneous coronary intervention (PCI) were consecutively included in this study. Plasma was collected from each participant on admission, the levels of plasma miR-208b-3p were measured using real-time PCR, and the N-terminal pro-brain natriuretic peptide (NT-proBNP) and hypersensitivity C reactionprotein (hsCRP) were detected by electrochemi-luminescence. Clinic SYNTAX score (CSS) was calculated by coronary angiography and clinical data and left ventricular ejection fraction (LVEF) was measured by improved Slimpson 's method of echocardiogram. The patients were followed up from the in-hospitals and those 3 years after PCI, respectively. The major adverse cardiovascular events (MACE) of hospitalization include death, heart failure, cardiogenic shock/hypotension, and malignant arrhythmia, while the MACE of 3 years follow-up includes death, heart failure, cardiogenic shock/ hypotension, malignant arrhythmia, recurrent myocardial infarction, and LVEF<50%. Prognostic accuracy of the plasma miR-208-3p was evaluated based on receiver operating characteristic curve (ROC) and area under curve (AUC). The prognostic accuracy of miR-208b-3p on MACE was also compared to that of hsCRP, NT-proBNP, CSS, or LVEF.
The plasma miR-208b-3p level was significantly higher in the MACE group than that in the non-MACE group (92.42±17.19 vs 15.07±3.12, p<0.001) and was positively correlated with CSS (r =0.212, p=0.032). MiR-208b-3p (AUC=0.767, p<0.001), NT-proBNP (AUC=0.654, p=0.015) and CSS (AUC=0.632, p=0.023) were strongly associated with increased risk of MACE within 3 years. Multivariate logistic regression analysis showed that Odds Ratio (95% confidence interval), adjusted for Nt-proBNP and CSS were 1.036 (1.101-1.062, p=0.006) for miR-208b.
Plasma miR-208b-3p is an independent predictor of MACE of 3 years follow-up and could be a useful biomarkers for predicting prognosis.