Author + information
- Konstantinos Maniatis,
- Gerasimos Siasos,
- Dimitris Tousoulis,
- Evangelos Oikonomou,
- Marina Zaromitidou,
- Stamatios Kioufis,
- Antigoni Miliou,
- Charalambos Vlachopoulos,
- Manolis Vavuranakis,
- Constantina Aggeli,
- Aris Plastiras,
- Athanasios Papavassiliou and
- Christodoulos I Stefanadis
Osteoprotegerin (OPG) and osteopontin (OPN) are bone metabolism biomarkers which are involved in the regulation of vascular calcification processes. We examined the relation between serum levels of OPG and OPN, vascular function and the severity of coronary artery disease (CAD).
The study population consisted of 409 subjects undergoing diagnostic coronary angiography for suspected CAD. Subjects were angiographically categorized in the group of CAD: 280 patients (mean aged 61±11years), and control group: 129 healthy subjects (mean aged 60±12 years). Gensini score was used to evaluate the severity of CAD based on the narrowing of the lumen diameter, the site and the numbers of the stenotic vessels, with greater values representing extensive CAD. Values of Gensini score>80 were consider to reflect severe CAD. Serum OPG and OPN levels were measured using ELISA. Endothelial function was evaluated by flow mediated dilation (FMD) in the brachial artery and carotid-femoral pulse wave velocity (PWV) was measured as an index of aortic stiffness.
CAD patients had significantly impaired FMD (p<0.001), increased PWV (p=0.006), increased OPG (p<0.001) and logOPN levels (p<0.001) compared to control subjects. PWV was positively associated with serum OPG levels (r=0.19, p<0.001) and FMD was inversely correlated with Gensini score (r=-0.22, p<0.001). Moreover, OPG (r=0.19, p<0.001) and logOPN (r=0.18, p=0.001) were positively associated with Gensini score. Importantly, OPG and OPN (p=0.03 for both) were also associated with Gensini score even after adjustment for age, sex, the presence of diabetes mellitus, dyslipidemia, arterial hypertension and smoking habits. Moreover, ROC curve analysis revealed that OPN has sufficient discriminate ability to detect severe CAD (Area Under the curve=0.635, 95%CI 0.524 to 0.746, p=0.03), and for an OPN value of 60.75 pmol/l there is a sensitivity of 65% and a specificity of 55%.
Our findings indicate that OPG and OPN serum levels are associated with vascular dysfunction and coronary atherosclerosis. Moreover, increased OPG and OPN levels are associated with the severity of CAD and increased OPN levels can predict severe CAD.
Poster Sessions, Expo North
Saturday, March 09, 2013, 3:45 p.m.-4:30 p.m.
Session Title: What's New with Risk Stratification in SIHD: Biomarkers, Genes and ECG
Abstract Category: 10. Chronic CAD/Stable Ischemic Heart Disease: Clinical
Presentation Number: 1154-70
- 2013 American College of Cardiology Foundation