Author + information
- Ali Faramarzalian,
- David Prabhu,
- Ahmad Abdul–Aziz,
- Daniel Chamie,
- Hirosada Yamamoto,
- Yusuke Fujino,
- Wei Wang,
- Nicholas Ziats,
- Andrew Rollins,
- David Wilson,
- Marco Costa and
- Hiram Bezerra
We introduce a novel methodology for volumetric comparison of images from intravascular optical coherence tomography (OCT) versus cryoimaging and histology. We also evaluate the capacity of cryoimaging to characterize fibrous cap inflammation in atherosclerotic plaques.
Ten cadaveric coronaries were screened by OCT for fibroatheromatous and fibrous plaques. Cryoimaging and histology were then executed for each vessel (figure). Fibrous cap area and percent macrophage were measured from trichrome and antiCD68 slides. Fibrous caps were stratified into inflamed or non–inflamed groups based on two thresholds: 0% and 10% macrophage. Images from cryoimaging were split into single channels and intensity was measured over the pixel area of the cap. Univariate and multivariate logistic regression with stepwise selection were performed. Odds ratios (OR) and area under the receiver operating characteristic curves (AUC) were calculated.
OCT accurately detected 81% (9 of 11) and 86% (6 of 7) of fibroatheromatous and fibrous plaques versus histology. Green fluorescence was the best marker of inflammation at 0% (OR=0.93, p<0.05) and 10% (OR=0.95, p<0.05) macrophage with AUC of 0.958 (95% CI=0.869–1.000, p<0.001) and 0.790 (95% CI=0.516–1.000, p<0.05), respectively.
Cryoimaging is a feasible adjuvant for volumetric validation of plaque characterization by OCT. Cryoimaging is able to accurately characterize fibrous cap inflammation in ex vivo human coronaries.
West, Room 2004
Saturday, March 09, 2013, 9:00 a.m.–9:10 a.m.
Session Title: Translational Science
Abstract Category: 52. TCT@ACC–i2: Translation and Pre–clinical Research
Presentation Number: 2902–9
- 2013 American College of Cardiology Foundation