Author + information
Stem cell therapy has potential towards aiding dilated cardiomyopathy (DCM), however, the electromechanical physiology of host and graft interaction remains unknown. The study is determined to reveal the underlying mechanism with novel multiple-scale engineering modeling method.
The co-culture instrumentation was designed with the recording electrode/mechanical array and larger auxiliary electrodes in the centre well, which was used for stimulation or additional recording electrodes over a larger area. The reusable acrylic barrier defines two chambers. The novel micro electrode array, micro mechanical array, bioluminescence and fluorescence imaging technology have been used to analyze the profile of graft-host interaction in multi-scale modality.
To ensure uniformity of conduction with host cells, human bone marrow mesenchymal stem cells (hBMMSC, expressed with Luc-eGFP) were co-cultured with human cardiomyocytes derived from dilated cardiomyopathy patients who received heart transplantation. Following the removal of the barrier on day 1, both populations of cell cultures merged together within 24 h. Electrical signals observed at this point were confirmed as originating from the host side. Between days 5 and 7, additional action potentials were observed on the graft side (N=5), beating synchronously with the host, and exhibited a lower AP amplitude propagation velocity, significantly decreased from the host side to graft, 11.3±2.1 mm/sec V.S 1.5±0.6 mm/sec (Fig.1, p<0.05). Meanwhile, the bioluminescence and fluorescence imaging has shown the profiling of cell proliferation and cell fusion.
The multi-scale engineering modeling shed light on the new hope to reveal the electrophysiological interactions between cardiac graft and host. With further validation, it would become applicable as a preclinical screening approach on cell therapy and new emerging medications.
Poster Sessions, Expo North
Saturday, March 09, 2013, 10:00 a.m.-10:45 a.m.
Session Title: Arrhythmias: Basic Science and Atrial Fibrillation
Abstract Category: 5. Arrhythmias: Basic
Presentation Number: 1106-43
- 2013 American College of Cardiology Foundation