Author + information
- Ahmet Sayın1,
- Burcu Zihni1,
- Mustafa Beyazıt Alkan1,
- Murat Bilgin5,
- Berkir Serhat Yıldız3,
- İlker Gül4,
- Muhsin Özgür Çoğulu2,
- Emin Karaca2 and
- Mehdi Zoghi1
The relationship of miRNA levels in patients admitted to the hospital because of acute decompensated heart failure (ADHF) as cause of heart failure, in hospital and short-term cardiovascular events, hospital readmission and mortality.
Our study included 47 patients with decompansated HF (10 non-ischemic and 37 ishemic origin) and 30 healty subjects (mean age: 57.13±8.32, %46.6 men). Peripheral blood was withdrawn and kept under appropriate circumstances to evaluate miR-22, miR-24 and miR-92b levels. When sample collection was completed, the levels of mRNA detected from peripheral blood cells (leukocytes) were calculated using the method PCR. The fold differences between groups were calculated as average and miRNA ▵Ct(the amount of normalized miRNA in a cycle) values were used for comparison.
%63.8 of the patients were male and mean age was 61.70±11.75. 39 patient had left ventricular systolic dysfunction and the average LVEF was %26.66±7.19. All patients', in-hospital mortality was %6.3, 1-month mortality rate was %4.5 and in this duration hospital readmission rate was %31.8. There was a significant fold change (>2 fold) between the patients miR-22, miR-24 and miR-92b levels and the control group. However, the significant changes in miRNA, both in low and preserved left ventricular systolic function or in terms of etiology of HF showed no difference. Similarly, there was no correlation between the ▵Ct values in terms of in-hospital and short-term cardiovascular endpoints.
Although there was a significant fold change in miRNA levels in patients hospitalized for ADHF, it did not correlate with the clinical endpoints analysed with ▵Ct values.
|Gender (m/f)||30 / 17|
|LVEF (all HF group's average)||%30.40±10.4|
|SVEF (LVD group)||%26.66±7.19|
|PEF-KY (n)||8 (%17)|
|DEF-KY (n)||39 (%83)|
HF: Heart failure, BMI: body mass index, HT: hypertension, HLP: hyperlipidemia, DM: diabetes mellitus LVEF: left ventricle ejection fraction LVD: left ventricle dysfunction, PEF: preserved ejection fraction LEF: low ejection fraction
|According to the normal at admission||According to the normal at discharge|
|miR- 22||- 2.39 f||- 1.23 f|
|miR- 24||- 2.07 f||- 1.59 f|
|miR- 92b||+ 2.33 f||+ 3.0 f|
miRNA (miR): microribonucleic acid