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Ischemia reperfusion and apoptosis both are serious complications of myocardial ischemia. Although the restoration of blood flow to an ischemic organ is essential to prevent irreversible tissue injury, reperfusion may augment's tissue injury in excess of that produced by ischemia alone. Restoration of blood flow to ischemic myocardium results in the ischemia reperfusion (I/R) injury.This study was undertaken to investigate the cardioprotective potential of goserline acetate in regional ischemia reperfusion and apoptosis of the male rat hearts.
Eighteen Swiss albino rats were randomized into 3 equal groups(6 in each group). Group 1 sham group, rat underwent the same anesthetic and surgical procedure as the control group except for surgical ligation of LAD, Group 2 control group, rats underwent surgical ligation of LAD and subjected to regional ischemia for 25 min and reperfusion for 40 min, Group 3 Goserelin acetate treated, rats underwent abdominal subcutaneous injection of Goserelin (LHRH analogue) 3wks before the surgery, then underwent surgical LAD ligation, and subjected to 25min of ischemia and 40min of reperfusion.At the end of experiment blood samples were collected from the heart for measurement of plasma level of cardiac troponin I(cTn I). The heart were harvested, and divided into 3 sections, the 1st for the measurement of cardiac apoptosis level, the 2nd homogenized for measurement of tissue (TNF-α,IL1-β,ICAM-1) and the 3rd was fixed in 10% formalin for histological examination.
Compared with the sham group, levels of myocardial TNF-α & IL-1β, ICAM-1 and apoptosis; plasma cTn I were increased (p<0.05) in control group. Histologically, all control group rats showed significant myocardial injury (p<0.05) compared with sham group. Goserelin significantly counteract the increase in myocardial level of TNF-α, IL-1, ICAM-1, plasma cTn I and apoptosis (p<0.05). Histological analysis revealed that Goserelin markedly reduced (p<0.05) the severity of heart injury in the rats underwent the regional ischemia- reperfusion procedure.
Goserelin acetate may ameliorate regional I/R injury and apoptosis in the ischemic heart in rats via interfering with inflammatory pathways.