Author + information
- Qiqiang Jie,
- Dongzhi Wang,
- Baoxin Liu,
- Yong Li,
- Jiachen Luo and
- Yidong Wei
To explore the age-related expression of calcium channels, Ca2+ handler proteins, hormone receptors and transcription factors in Ventricular Myocyte during mouse embryonic heart development.
By using real-time PCR assays to investigate the dynamic changes in the expression of calcium channels and related proteins in mouse ventricular myocytes from embryonic 12 days, embryonic 15 days, embryonic 18 days, postnatal 1 day and adulthood.
During mouse embryonic heart development, the expression of most proteins was increased along with heart development. On the one hand, the expression of several proteins, such as Cav1.2 (α1C), Cav1.3 (α1D), Cav3.2 (α1H), IP3R1, IP3R2, CaMKIId, Nkx2.5, GATA4 and Tbx5, was highest in embryonic 15 days. Expression of most proteins decreased around embryonic 18 days, postnatal 1 days and adulthood. However, Cav1.2 still maintained high expression in adulthood. On the other hand, the expression of proteins, including Cav3.1 (α1G), RyR2, Agtr1a and Agtr1b, increased during embryonic heart development, peaked at embryonic 18 days, and then Cav3.1 decreased sharply around postnatal 1 day and adulthood, but RyR2, Agtr1a and Agtr1b still have a high expression.
The expression of calcium channels, ca2+ handler proteins, hormone receptors and transcription factors in Ventricular Myocyte showed an age-related changes during mouse embryonic heart development. Most of these proteins expressed highest in embryonic 15 days and decreased subsequently.