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To investigate the paracrine effects of bone marrow mesenchymal stem cells (MSCs)-derived exosomes on angiogenesis and anti-inflammatory activity to improve heart function.
MSCs were cultured in DMEM/F12 supplemented with 10% exosome-depleted fetal bovine serum and 1% penicillin-streptomycin for 48 h. MSCs-derived exosomes were isolated using ExoQuick-TC. Tube formation and T-cell proliferation assays were performed to assess the angiogenic potency of exosomes. Acute myocardial infarction was induced in Sprague-Dawley rats. Phosphate-buffered saline (PBS, control), MSCs-derived exosomes, and exosome-depleted MSCs culture medium were injected at four different sites bordering the infarcted zone.
MSCs-derived exosomes significantly promoted tube formation of HUVECs compared with frozen MSCs-derived exosomes (15688.42 ± 8656.62 vs. 11343.64 ± 986.78, respectively, P < 0.05). A significant increase in new capillaries was observed in the MSCs-derived exosome group than in PBS (1267.35 ± 84.23 vs. 744.38 ± 93.88, P < 0.05) and exosome-depleted CM groups (1267.35 ± 84.23 vs. 647.38 ± 103.23, P < 0.05). Blood vessel density increased in heart injected with exosomes than with PBS (132.42 ± 11.78 vs. 46.81 ± 10.29, P < 0.05). There were decreased inflammatory cells in the exosome group than in PBS and exosome-depleted CM groups (P < 0.05).
MSCs-derived exosomes improve heart function after ischemic injury by stimulating neovascularization and restraining the inflammation response.