Author + information
- Mohammad Mathbout,
- Ahmed Asfour,
- Yuri Klyachkin,
- Himi Tripathi,
- Ahmed Abdel-Latif and
- Khaled Ziada
Background: Acute ST segment elevation myocardial infarction (STEMI) initiates multiple signaling mechanisms between the injured myocardium and bone marrow culminating in stem cell (BMSC) mobilization. These signaling pathways and their clinical significance are poorly understood. We have recently published a paper on the role of sphingosine-1 phosphate (S1P) in mobilizing BMSCs after acute cardiac injury.
Methods: Plasma levels of sphingosine-1 phosphate and circulating BMSCs were assessed in 76 patients with STEMI during the acute phase and at 6, 12, 24, and 48 hour intervals (Figure 1A). Levels of circulating Lineage negative/CD45 negative cells that are positive for CD133 and/or KDR-1 were examined using flow cytometry. Echocardiography and cardiac MRI were performed prior to hospital discharge. Patients were followed for major adverse cardiac events for a median of 12 months.
Results: Plasma S1P levels demonstrated dynamic changes following STEMI, peaking at 6 hours. Peak S1P correlated positively with circulating BMSCs such as Lin-/CD45-/CD133+ (r=0.2, P<0.05) and Lin-/CD45-/KDR-1+ BMSCs (r=0.2, P<0.05). BMSCs enriched in endothelial progenitors predicted clinical outcomes in our population with reduced adverse cardiac events among patients with higher numbers of circulating Lin-/CD45-/CD133+ and Lin-/CD45-/KDR-1+ (P<0.05).
Conclusions: Sphingosine-1 phosphate correlates with endothelial progenitor cell mobilization which predicts clinical outcomes in patients with STEMI.
Poster Hall, Hall C
Friday, March 17, 2017, 10:00 a.m.-10:45 a.m.
Session Title: Novel Mechanisms Underlying Ischemia-Reperfusion Injury in AMI
Abstract Category: 1. Acute and Stable Ischemic Heart Disease: Basic
Presentation Number: 1124-297
- 2017 American College of Cardiology Foundation