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To Establish the miniature pig carotid atherosclerotic model and analyze the effects of NLRP3 on the AS plaque stability and the relationship between the shearing force and the formation and stability of plaque.
8 miniature pigs were given high fat and high cholesterol diet for 2 weeks to induce hypercholesterolemia. Then take the partial carotid artery ligation. After the operation, keep on the high fat and high cholesterol feeding for 3 months to establish carotid atherosclerosis model. During the operation, 16 vascular stenosis formation were diagnosed by Doppler ultrasound in 70% pigs. The 1st, 2nd and 3rd month after the operation, Doppler ultrasound was used to observe the formation of carotid plaque and the progress of the shearing force. At the end of 3 months, get 351 4mm pieces from both of bilateral carotid artery vascular. HE staining was to determine the plaque classification. And applied the pathological detection to observe the expression of NLRP3 inflammasome at different levels of plaque. Furthermore, analyses the relationship of NLRP3 and the multiple characteristics of unstable plaque.
Serum apoB had inverse correlations with most of the LV structural and functional indices studied. High serum apoB level was significantly related to the decrease in left atrium dimensions (r = -0.20, P = 0.011), LV dimensions (end-diastolic diameter: r = -0.27, P = 0.001; end-systolic diameter: r = -0.24, P = 0.003), E/A ratio (r =-0.38, P < 0.001) and LV volumetric dimensions (end-diastolic volume: r = -0.27, P < 0.001; end-systolic volume: r = -0.28, P < 0.001). After adjustment for clinical confounding factors, the effect of serum apoB on LV remodeling (characterized by left ventricular end-diastolic volume dilatation) modestly weakened but remained statistically significant (adjusted r = -0.16, P = 0.038), while other associations disappeared. In multivariate linear regression analysis, conditional lipid profiles were not significantly associated with LV eccentric remodeling, whereas serum apoB was an independent determinant of LV dilatation.
In our PD population, serum apoB was significantly and inversely associated with LV dilatation, independently of conventional lipids and other CV risk factors. It suggests that low serum apoB concentrations may be a sensitive and powerful risk markers of eccentric left ventricular geometry remodeling, which might help to risk-stratify maintenance peritoneal dialysis patients.