Author + information
Macrophages play a central role in each stage of development of atherosclerosis (AS). Long chain noncoding RNAs (IncRNAs) has been proved to regulate macrophage function and foam cell formation. However, it is not clear whether LncRNAs plays a role in regulating the function of macrophages in the process of stabilizing the AS plaque to the ruptured AS plaque.
Using bioinformatics method to re-annotate gene chip (GSE41571), construct co-expression networks of differentially expressed lncRNA and mRNA, predict and confirm target molecule lncRNA-CRNDE according to its function. The effect of lncRNA-CRNDE on the function of macrophages was verified by RNA interference in human THP-1macrophage.
It was found that there were significant differences in the expression of 17 IncRNAs genes in the macrophages in the stable AS plaques and in the ruptured AS plaques. Among these, LncRNA-CRNDE and matrix metalloproteinase (MMPs) -2 and MMP9 were significantly co-expressed. Stimulation of macrophages with LPS or ox-LDL could significantly increase the expression of lncRNA-CRNDE,and there was dose effect between lncRNA-CRNDE expression and ox-LDL. The mRNAand protein expression ofMMP-9 and MMP-2 were significantly decreased when lncRNA-CRNDE was silenced.
There was difference in IncRNAs in macrophages between stable AS plaques and ruptured AS plaques. IncRNA-CRNDE may regulate the expression of MMP-2/MMp-9 and influence the function of macrophage in AS plaque rupture.