Author + information
- Theodoros Kelesidis,
- Elizabeth Tran,
- Karishma Lakhani,
- Sara Arastoo,
- Rachel Heymans and
- Holly Middlekauff
Electronic cigarettes (ECs), advertised as a safer alternative to tobacco cigarettes (TCs), are increasingly used by young, never smokers (NS). TCs increase oxidative stress (OS) leading to premature atherosclerotic cardiovascular disease (CVD), but the effects of ECs on OS remain poorly understood. We aimed to assess the effects of chronic EC use and TC smoking on cellular OS (COS) in humans.
In 11 non-smokers (NS; age 24.5 ± 2.0 y, 7 M), 12 chronic EC users (24.1 ± 4.1 y, 8 M) and 9 chronic TC smokers (24.9 ± 3.8 y, 4 M) blood was assessed by flow cytometry for levels of COS. Two readouts of COS were used: i) % of cells that were positive for the fluorochrome CellROX Green (that determines the total cellular content of ROS); ii) the median cellular amount of ROS per cell type [median fluorescence intensity (MFI) of CellROX Green per cell type per sample minus the MFI of negative staining control]. Cellular ROS was determined in neutrophils (PMNs) and monocytes (MNCs) that carry abundant ROS and contribute to systemic OS and CVD. The Mann-Whitney and the Kruskal-Wallis statistical tests were used.
Both the % of CellROX+ cells and CellROX MFI increased in proinflammatory MNCs in chronic TC smokers and chronic EC users compared to NS. EC users and TC smokers did not differ in COS in PMNs and classical MNCs.
The increase in cellular oxidative stress in proinflammatory MNCs is similar in chronic TC smokers and EC users compared to non-smokers, potentially leading to future CVD.
Posters Hall_Hall A
Monday, March 30, 2020, 12:30 p.m.-1:15 p.m.
Session Title: Vascular Medicine: Basic 8
Abstract Category: 38. Vascular Medicine: Basic
Presentation Number: 1468-204
- 2020 American College of Cardiology Foundation